CD-spectroscopy is a technique that allows for investigating molecular structure of a material by comparing its absorption of left- and right-handed circularly polarized light. By examining how different wavelengths of circularly polarized light are absorbed by a sample, CD spectroscopy reveals details about the molecule’s shape, folding, and interactions. Exemplary applications of CD-spectroscopy are:
• Chiral analysis of chemical compounds
• Structural analysis of polymers and copolymers
• Protein secondary structure and their folding / unfolding processes as well as interactions
• Nucleic acid conformation
• Enzymatic interactions

The samples should be soluble in common organic solvents, e.g. chloroform, THF, etc., or water. The method requires as little as 10 mg of the material. However, for the sake of manipulation, we would ask for at least 100 mg. We understand that certain substances can be achieved only on ng scale. If it’s your case, get in contact with us and we find a common solution. The samples can be delivered as solid bulk, powder, liquid or already prepared solution in a solvent of a proper purity. Analysed materials can be organic compounds, polymers, proteins, nucleic acids, enzymes and many more. If you don’t know this about your sample, ask us !

A standard report includes raw data and experimental spectrum. If you are looking for a in-depth analysis, ask us !

Spatial structure of molecules grants some of them a feature of chirality, inability to be superimposed onto its mirror projection. Such molecules cause absorb differently left- and right-handed circularly polarised light. A thin solution of the studied compound is placed into the spectrometer. Differently circularly polarized light is passed through the sample and the differential absorption is measured. The difference in absorption of left- and right-handed circular polarised light is the circular dichroism. Comparison of circular dichroism at different wavelengths provides information that can be interpreted as the molecular structure.