Fluorescence spectroscopy is an analytical technique, in which information about molecular structure of the molecules is achieved through their excitation to higher energy levels and measuring their emission spectra. It enables the quantitative analysis of samples by measuring the intensity of fluorescence emitted by specific fluorophores, measuring concentration of emitting substances. The information received can be bulk structure, conformation, interaction, or environmental effects in the studied sample. It is typically performed with substances marked with fluorescent dyes or fluorophores. However, certain molecules, e.g. some proteins, are naturally capable of fluorescence. Exemplary applications include:
• Interactions of (bio)molecules as DNA, protein, enzymes, drugs, polymers, particles etc.
• Conformational changes in proteins
• Drug screening
• Cellular imaging
• Pollutants monitoring
• Structure and conformation of polymers

The studied sample should contain fluorescent molecules. These can be introduced separately or belong to the studied sample. The sample should be soluble or suspensible in water or a common organic solvent e.g. chloroform, THF, etc. The method requires as little as 10 mg of the material. However, for the sake of manipulation, we would ask for at least 100 mg. We understand that certain substances can be achieved only on ng scale. If it’s your case, get in contact with us and we find a common solution. The samples can be delivered as solid bulk, powder, liquid or already prepared solution in a solvent of a proper purity. Analysed materials can be organic compounds, polymers, proteins, nucleic acids, enzymes and many more. If you don’t know this about your sample, ask us !

A standard report includes raw data and experimental spectrum. If you are looking for a in-depth analysis, ask us !

Certain molecules are capable of fluorescence, an ability to emit light of a particular wavelength after absorption of energy. In practice, a sample is irradiated with a broad spectrum light that is absorbed by the fluorescent molecules. Then, the excited molecules lose a part of the absorbed energy through non-radiative processes as heat dissipation and collisional relaxation. This brings them to a lower excited state, vibrational or rotational level. Further relaxation havens via radiative process called fluorescence. The energy of the emitted photons is unique for each fluorescent molecule. This allows for precise identification of the chemical structure and conformation of the studied sample, while the intensity of the signal provides information of concentration of fluorescent moieties. However, only having knowledge of possible sample composition, i.e. structure of the fluorescent moieties, it is possible to provide reliable analysis of the spectroscopical data.